H9286

Immunoblot
Whole cell extract from serum and sodium butyrate-treated NIH3T3 cells was separated on SDS-PAGE and blotted with Anti-Acetyl-Histone H3 [Ac-Lys9] (Cat. No. H9286). The antibodies were developed with Goat Anti-Rabbit IgG-Alkaline phosphatase (Cat. No. A9919) and SIGMA FAST™ BCIP/NBT substrate (Cat. No. B5655). Inhibition with the acetylated [Ac-Lys9] histone H3 peptide (Lane 2) with no inhibition with the non-acetyl-histone H3 peptide (Lane 3).
Lane 1: Anti-Acetyl-Histone H3 [Ac-Lys9]
Lane 2: Anti-Acetyl-Histone H3 [Ac-Lys9] + [Ac-Lys9] histone H3 peptide
Lane 3: Anti-Acetyl-Histone H3 [Ac-Lys9] + [non-Ac] histone H3 peptide

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H9286

Sigma

 

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:MFCD02685921

Description

Immunogensynthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formaffinity isolated antibody
clone(polyclonal)
formbuffered aqueous solution
mol wtantigen mol wt ~17 kDa
species reactivityDrosophila, human, bovine, chicken, frog, Caenorhabditis elegans, rat, mouse
application(s)immunoblotting: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate
 indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
 microarray: suitable
Gene Informationhuman ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
 rat ... H3f3b(117056)
 mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
shipped indry ice
storage temp.−20°C

Safety

WGK GermanyNWG

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References

referenceBadley-Clarke, J., et al., DOK1 mediates SHP-2 binding to the αVβ3 integrin and thereby regulates insulin-like growth factor I signaling in cultured vascular smooth muscle cells. J. Biol. Chem. 280, 3151-3158, (2005) Abstract
 Clemmons, D., et al., p66shc Negatively regulates insulin-like growth factor I signal transduction via inhibition of p52shc binding to Src homology 2 domain-containing protein tyrosine phosphatase substrate-1 leading to impaired growth factor receptor-bound protein-2 membrane Mol. Endocrinol. 22, 2162-2175, (2008) Abstract
 Clemmons, D.R., et al., Role of SHPS-1 in the Regulation of Insulin-like Growth Factor I-stimulated Shc and Mitogen-activated Protein Kinase Activation in Vascular Smooth Muscle Cells Mol. Biol. Cell 16, 3353-3364, (2005) Abstract